Analysis Note

ControlRat Brain lysate

Application

Immunohistochemistry: 1:100 working dilutions were used on rat and mouse brain (paraffin embedded tissue).

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Anti-Tyrosine Hydroxylase Antibody detects level of Tyrosine Hydroxylase & has been published & validated for use in IH(P) & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tyrosine Hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Immunogen

Recombinant protein

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3

Format: Purified

Antigen Affinity Purified

Specificity

Cat. # AB9983 recognizes the C-terminus of Tyrosine Hydroxylase

Reactivity with other species has not been determined.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 58 kDa